Identificiaton of differentially methylated and mutated nucleic acids
AssigneeNorth Shore-Long Island Jewish Research Institute
InventorHao-Peng Xu Duffy
Life of this patent
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Abstract
The present invention provides Methyl- (or Mutant-) Differential Display (MDD) methods and nucleic acid probes for detecting mutations and the methylation patterns of nucleic acids. Regions of the genome are differentially methylated or mutated in different cell types, including cancerous cell types. The TSP50 gene is thereby identified and found to be differentially expressed in breast cancer cells. The present invention provides methods and compositions for identifying aberrant expression of the TSP50 gene and of tsp50 protein. Antibodies of the present invention can be used for diagnosis and treatment of diseases characterized by aberrant tsp50 expression.
Claims25
An isolated antibody which binds selectively to tsp50 protein comprising the amino acid sequence of SEQ ID NO:16.
A method of producing antibodies which bind to tsp50 protein comprising the amino acid sequence of SEQ ID NO:16, which comprises:
- a) immunizing an animal with a suitable amount of tsp50 protein or a polypeptide fragment thereof;
- b) collecting the serum from the animal;
- and
- c) isolating the tsp50-specific immunoglobulins from the serum of said animal.
A method of producing a hybridoma which secretes an antibody that binds to tsp50 protein comprising the amino acid sequence of SEQ ID NO:16, which comprises:
- a) immunizing an animal with tsp50 protein or a polypeptide fragment thereof;
- b) obtaining lymphoid cells from the immunized animal;
- c) fusing the lymphoid cells with an immortalizing cell to produce hybrid cells;
- and
- d) selecting hybrid cells which produce antibody that specifically binds to tsp50 protein.
A method of producing an antibody that binds to tsp50 protein comprising the amino acid sequence of SEQ ID NO:16, which comprises:
- a) synthesizing a library of antibodies on phage;
- b) panning the library against a sample by bringing the phage into contact with a composition comprising tsp50 or a polypeptide fragment thereof;
- c) isolating phage which bind tsp50;
- and
- d) obtaining an antibody from the phage.
A kit for specific assay of tsp50 protein in a test sample comprising an antibody or antibody fragment capable of binding specifically to tsp50 protein comprising the amino acid sequence of SEQ ID NO:16.
Dependencies are inferred from the claim text.