Nucleic acid molecule encoding an ankyrin repeat TVL-1 protein and methods of use thereof
AssigneeThomas Jefferson University
InventorsPhilip N. Tsichlis, Antonios Makris
Life of this patent
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Abstract
Nucleic acid molecules, proteins and antibodies are provided which may be used in biological screens to identify therapeutic agents involved in the regulation of cell cycle progression and apoptotic cell death.
Claims8
An isolated protein between about 250 and 270 amino acids in length, the protein comprising a plurality of ankyrin repeat domains and capable of promoting TNFα-induced apoptosis, wherein said protein is a product of expression of part or all of the nucleic acid of SEQ ID NO:1.
An isolated polypeptide having a part of amino acids set forth in SEQ ID NO:2 or all of amino acids set forth in SEQ ID NO:2 or encoded by a nucleic acid molecule having a sequence selected from the group consisting of:
- (a) a sequence set forth in SEQ ID NO:1 or a fragment thereof;
- (b) a sequence hybridizing under stringent conditions with part or all of the complementary strand of SEQ ID NO:1, and
- wherein said conditions include incubation at 37-42° C. for at least six hours in a hybrization solution comprising:
- 5×SSC, 5×Denhardt's reagent, 1.0% SDS, 100 mg/ml denatured, fragmented salmon sperm DNA, 0.05% sodium pyrophosphate and up to 50% formamide and wherein the isolated polypeptide is capable of interacting with Raf-1 and capable of promoting TNFα-induced apoptosis.
An isolated polypeptide encoded by a nucleic acid molecule having a sequence set forth in SEQ ID NO:1.
An isolated protein having an amino acid sequence the same as that set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.