Serum free medium for chondrocyte cells
AssigneesConsorzio per la Gestione del Centro di Biotechnologie Avanzate, Istituto Nazionale per la Ricerca Sul Cancro
InventorsRanieri Cancedda, Beatrice Dozin
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Abstract
Serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum free medium for growth of chondrocytes includes a serum free composition comprising FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin and dexamethasone. Further the composition comprises EGF, PDGFbb, insulin and albumin. A method for growing chondrocytes in a serum free medium comprising the composition is also provided. Also provided for mesenchymal stem cell growth, is a serum free medium which includes a composition comprising FGF-2, LIF, SCF, pantotenate, biotin and selenium and method, therefore.
Claims6
A serum-free culture medium for chondrocytes, comprising FGF-2, linoleic acid, ascorbic acid, β-mercaptoethanol, transferrin and dexamethasone.
A method for culturing chondrocytes, which comprises growing cells in a serum-free composition comprising FGF-2, linoleic acid, ascorbic acid, β-mercaptoethanol, transferrin and dexamethasone.
Dependencies are inferred from the claim text.