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US 6,617,148 B2Granted
Natural promoters for gene expression and metabolic monitoring in bacillus species
AssigneeE. I. Du Pont de Nemours and Company
InventorsLaura A. Bedzyk, Tao Wang, Rick Ye
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Abstract
Genes have been identified in the Bacillus genome that are responsive to various metabolic conditions and growth cycle changes. The new responsiveness of these genes allows for their use in regulated gene expression in Bacillus sp. and for the monitoring of bioreactor health.
Claims7
A method for the expression of a coding region of interest in a Bacillus sp comprising:
- a) providing a transformed Bacillus sp cell having a chimeric gene comprising a nucleic acid fragment comprising the promoter region of a Bacillus gene operably linked to a coding region of interest expressible in a Bacillus sp, wherein the nucleic acid fragment comprising the promoter region of a Bacillus gene is narGHJI;
- and
- b) growing the transformed Bacillus sp cell of step (a) in the absence of oxygen wherein the chimeric gene of step (a) is expressed.
A method for the expression of a coding region of interest in a Bacillus sp comprising:
- a) providing a transformed Bacillus sp cell having a chimeric gene comprising a nucleic acid fragment comprising the promoter region of a Bacillus gene operably linked to a coding region of interest expressible in a Bacillus sp, wherein the nucleic acid fragment comprising the promoter region of a Bacillus gene is narGHJI,;
- b) growing the transformed Bacillus sp. cell of step (a) in the presence of oxygen whereby the cell density is increased;
- and
- c) removing oxygen from the transformed Bacillus sp. cell or step (b) whereby the chimeric gene is expressed.
1 more element
Dependencies are inferred from the claim text.