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US 6,617,135 B1Granted
Multiple cytokine protein complexes
AssigneeEMD Lexigen Research Center Corp.
InventorsStephen D. Gillies, Kin-Ming Lo
Life of this patent
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Abstract
The invention relates to protein complexes and fusion proteins including at least two different cytokine molecules. The protein complexes and fusion proteins may further include a targeting moiety such as a region of an immunoglobulin. Methods of using the protein complexes and fusion proteins are also disclosed.
Claims17
A fusion protein comprising:
- a first polypeptide chain comprising an immunoglobulin region and a first subunit of interleukin-12 (IL-12);
- and
- a second polypeptide chain comprising a cytoline and a second subunit of IL-12, wherein
- the cytokine is a four-helix bundle protein.
1 more element
A method of preparing a fusion protein comprising IL-12, a second, different cytokine, and an immonoglobulin region capable of targeting to a preselected locus in a mammal, the method comprising the steps of:
- (a) expressing in a host cell one or more nucleic acids encoding a fusion protein comprising IL-12, a second, different cytokine and an immunoglobulin region capable of targeting said fusion protein to a preselected locus when said fusion protein is administered to a mammal, wherein said second, different cytokine is a four-helix bundle protein;
- and
- (b) harvesting said fusion protein.
Dependencies are inferred from the claim text.