Process for purifying recombinant human serum albumin
AssigneeMitsubishi Pharma Corporation
InventorsMunehiro Noda, Akinori Sumi, Takao Ohmura, Kazumasa Yokoyama
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Abstract
The invention provides a process for purifying recombinant human serum albumin (rHSA) by heating a-culture medium containing rHSA and the rHSA-producing host cells, feeding said heated solution upwardly into a fluidized bed in which adsorbent particles are suspended to effect contacting with the adsorbent particles and then recovering the adsorbed fraction containing the rHSA, and a composition comprising rHSA which shows a A350/A280 ratio of below 0.015, when formulated into a 25% solution of said albumin.
Claims9
A process for purifying recombinant human serum albumin comprising:
- (a) heating a culture medium containing said albumin and recombinant human serum albumin-producing host cells in which said albumin has been produced to inactivate a protease in said culture medium;
- (b) contacting said heated culture medium with adsorbent particles suspended in a fluidized bed under conditions for selectively adsorbing said albumin;
- and
- (c) recovering the adsorbed purified fraction.
Dependencies are inferred from the claim text.