Nucleic acid molecule encoding the potassium channel protein, KCNQ5
AssigneeAventis Pharma Deutschland GmbH
InventorsKlaus Steinmeyer, Christian Lerche, Constanze Scherer, Guiscard Seebohm, Andreas E. Busch
Life of this patent
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Abstract
KCNQ5, DNA encoding KCNQ5 or a polypeptide having KCNQ5 activity, related vectors, host cells and methods and a screening assay for identification of compounds which modify activity of a potassium channel KCNQ5 are described.
Claims17
An isolated nucleic acid molecule encoding a potassium channel protein, KCNQ5, polypeptide comprising SEQ ID NO:2.
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising SEQ ID NO:
- 1;
- (b) a nucleic acid molecule that hybridizes with a polynucleotide under conditions consisting of washing filters in 0.1×SSC, 0.1% SDS;
- 2 times for about 30 minutes at about 65° C. or about 5° C. below melting temperature, wherein the polynucleotide encodes a polypeptide which has substantially the same KCNQ5 activity as the polypeptide encoded by SEQ ID NO:
- 1;
- (c) a nucleic acid molecule encoding a polypeptide of SEQ ID NO:
- 2
- (d) a nucleic acid molecule that hybridizes to the nucleic acid molecule of one of (a)-(c), under conditions consisting of washing filters in 0.1×SSC, 0.1% SDS;
- 2 times for about 30 minutes at about 65° C. or about 5° C. below melting temperature;
- (e) a nucleic acid molecule that is degenerated from SEQ ID NO:
- 1, wherein said nucleic acid molecule encodes the KCNQ5 polypeptide of SEQ ID NO:
- 2 which has substantially the same biological activity as potassium channel protein, KCNQ5;
- and
- (f) a nucleic acid molecule that is complementary to the nucleic acid molecule of one of (a)-(e).
A cell culture for screening agonists and antagonists of potassium channel protein, KCNQ5, comprising cells that express KCNQ5, wherein KCNQ5 is encoded by the nucleic acid molecule of SEQ ID NO:
- 1.
A kit comprising one or more containers, wherein at least one container contains a detectably labeled nucleic acid probe that hybridizes with a polynucleotide encoding SEQ ID NO:
- 2 polynucleotide under conditions consisting of washing filters in 0.1×SSC, 0.1% SDS;
- 2 times for about 30 minutes at about 65° C. or about 5° C. below melting temperature.
Dependencies are inferred from the claim text.