Specific oligonucleotide primers for detection of bovine male chromosome presence by polymerase chain reaction and method
AssigneeXy, LLC
InventorAlan D. Dean
Life of this patent
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Abstract
This invention is a specific set of oligonucleotide Polymerase chain reaction (PCR) primers [5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1) and 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2)] for specific polymerase chain reaction (PCR) amplification of a region of the bovine male-specific chromosome sequence.
Claims16
A polymerase chain reaction primer the nucleotide sequence of which consists of 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID NO:1).
A polymerase chain reaction primer the nucleotide sequence of which consists of 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID NO:2).
A primer pair used for amplifying DNA of the bovine TSPY gene by polymerase chain reaction, wherein the primer pair comprises a first primer comprising the nucleotide sequence of 5′-TGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1), and a second primer the nucleotide sequence of 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2).
A kit useful to detect the presence of a portion of DNA sequence from Y chromosome, comprising:
- a a first container containing a first oligonucleotide primer comprising the nucleotide sequence 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1), wherein said first oligonucleotide primer binds to a specific Y chromosome polynucleotide sequence;
- and
- b at least a second container containing a reagent useful in performance of a polyrnerase chain reaction amplification.
A kit useful to detect the presence of a portion of DNA sequence from Y chromosome, comprising:
- a a first container containing a second oligonucleotide primer comprising the nucleotide sequence 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2), wherein said second oligonucleotide primer binds to a specific Y chromosome polynucleotide sequence;
- and
- b at least a second container containing a reagent useful in performance of a polymerase chain reaction amplification.
A method of detecting a specific Y chromosome sequence by polymerase chain reaction, comprising the steps of:
- a amplifying a specific Y chromosome polynucleotide sequence with a primer pair, wherein said primer pair comprises a first primer comprising the nucleotide sequence of 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1) and a second primer comprising the nucleotide sequence of 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2);
- and
- b detecting the polynucleotide product of said step of amplifying said specific Y chromosome polynucleotide.
A method of identifying the gender of an animal, comprising the steps of:
- a obtaining a meat sample from said animal;
- b amplifying a target nucleic acid sequence in said meat with a primer comprising the nucleotide sequence of 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1);
- c isolating amplified products from said genetic material;
- d determining the existence of Y chromosome polynucleotide sequence in said meat.
A method of amplifying a specific Y chromosome polynucleotide sequence by polymerase chain reaction, comprising the steps of:
- a establishing a specific Y chromosome polynucleotide sequence in a solution;
- b providing a primer pair, wherein said primer pair comprises a first primer comprising the sequence of 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1) and a second primer comprising the sequence of 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2) in said solution;
- c denaturing said specific Y chromosome sequence;
- d annealing each of said primer pair to a specific region on said specific Y chromosome sequence;
- and
- e extending each of said primer pair on said specific Y chromosome sequence using said specific Y chromosome sequence as a template to amplify a portion of said Y chromosome sequence.
A method of identifying the gender of an animal by polymerase chain reaction, comprising the steps of:
- a establishing a DNA material from a sample of meat of said animal in a solution;
- b providing a primer pair, wherein said primer pair comprises a first primer comprising the sequence of 5′-GTGATCCGGCATATAGCTGAGA-3′ (SEQ ID No. 1) and a second primer comprising the sequence of 5′-TGGTCGCTGATCAGGATGGAA-3′ (SEQ ID No. 2) in said solution;
- c denaturing said DNA material to produce two single stranded DNA sequences;
- d annealing each of said primer pair to a specific region on each of said single stranded DNA sequences which sequences are complementary to each of said primer sequences;
- e extending each of said primer pair on each of said single stranded DNA sequences using each of said single stranded DNA sequences as templates to amplify a specific region of each of said single stranded DNA sequences;
- f repeating said steps of denaturing said DNA material to produce two single stranded DNA sequences;
- annealing each of said primer pair to a specific region on each of said single stranded DNA sequences which sequences are complementary to each of said primer sequences;
- extending each of said primer pair on each of said single stranded DNA sequences using each of said single stranded DNA sequences as templates to amplify a specific region of each of said single stranded DNA sequences;
- and
- g detecting the presence of said specific regions from said DNA material wherein said specific regions have been amplified following said steps of c, d and e and wherein the presence of said specific regions from said DNA material is indicative of male genomic material in said DNA material.
Dependencies are inferred from the claim text.