Solid-phase nucleic acid isolation
AssigneeGenpoint AS
InventorsKnut Rudi, Kjetill Sigurd Jakobsen
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Abstract
The present invention provides a method of isolating nucleic acid from a sample of cells, said method comprising: (a) binding cells in said sample to a solid support to isolate cells from the sample; (b) lysing the isolated cells; and (c) binding nucleic acid released from said lysed cells to said same solid support and a kit for carrying out such a method. The method may advantageously be used to prepare nucleic acid for use in a nucleic acid-based target cell detection method.
Claims19
A method of isolating nucleic acid from a sample of cells, said method comprising:
- (a) binding cells in said sample onto a solid support coated with cell-binding moieties;
- (b) lysing the isolated cells;
- (c) binding nucleic acid released from said lysed cells to a solid support that consists of the same solid support of step (a);
- and
- (d) recovering nucleic acid from said support.
A method of isolating nucleic acid from a sample of cells, said method comprising:
- (a) binding cells in said sample onto a solid support coated with cell-binding moieties;
- (b) lysing the isolated cells;
- (c) binding nucleic acid released from said lysed cells to a solid support that consists of the same solid support of step (a);
- and
- (d) detecting said bound nucleic acid.
A method of isolating nucleic acid from a sample of cells, said method comprising:
- (a) binding cells in said sample to a solid support comprising magnetic beads to isolate cells from the sample;
- (b) lysing the isolated cells;
- (c) binding nucleic acid released from said lysed cells to a solid support that consists of the same solid support of step (a);
- and
- (d) recovering nucleic acid from said support.
A method of isolating nucleic acid from a sample of cells, said method comprising:
- (a) binding cells in said sample to a solid support comprising magnetic beads to isolate cells from the sample;
- (b) lysing the isolated cells;
- (c) binding nucleic acid released from said lysed cells to a solid support that consists of the same solid support of step (a);
- and
- (d) detecting said bound nucleic acid.
Dependencies are inferred from the claim text.