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US 6,613,895 B1Granted
System and methods for the rapid isolation of nucleic acids
AssigneeBio 101
InventorsJames Gautsch, Mark Brolaski
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Abstract
The present invention contemplates a system for rapidly isolating nucleic acids. The system comprises an insoluble silica matrix and a buffered aqueous salt solution containing salt at a concentration of at least 3 molar and a buffering agent at a concentration sufficient to provide a buffering capacity corresponding to that which either tris(hydroxymethyl)aminomethane or phosphate ion at a concentration of 0.1 to 1 molar would provide in the solution. Methods of using the system are also contemplated.
Claims17
A method for isolating nucleic acid from a sample containing nucleic acid, which method comprises:
- a) forming a binding reaction admixture by admixing said sample with an insoluble silica matrix and an aqueous salt solution, said insoluble silica matrix comprising particulate glass having a sedimentation time through 100 centimeters (cm) of still water at unit gravity in the range of 6 weeks to 20 minutes, said solution containing a salt at a concentration of at least 3 molar;
- b) maintaining said binding reaction admixture for a time period sufficient for said nucleic acid to bind to said matrix to form an insoluble nucleic acid-matrix complex and a remaining admixture;
- c) separating said remaining admixture and said complex to form an isolated complex;
- and
- d) recovering said nucleic acid from said isolated complex to form isolated nucleic acid.
2 more elements
Dependencies are inferred from the claim text.