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US 6,613,552 B1Granted
Biocatalytic synthesis of shikimic acid
AssigneeBoard of Trustees operating Michigan State University
InventorsJohn W. Frost, Karen M. Frost, David R. Knop
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Abstract
A bioengineered synthesis scheme for the production of shikimic acid from a carbon source is provided. Methods of producing shikimic acid from a carbon source based on the synthesis scheme are also provided.
Claims42
A method for the production of shikimic acid from a carbon source comprising:
- a) providing a microbe selected from the group consisting of bacteria, fungi and yeast, wherein the microbe comprises one or more enzyme-encoding recombinant DNA molecules, wherein the encoded enzyme is selected from the group consisting of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and 3-dehydroshikimate dehydrogenase, and wherein the microbe further comprises an inactivating mutation in at least one DNA molecule which encodes a shikimate kinase isozyme;
- and
- b) culturing said microbe in the presence of the carbon source.
A method for the production of shikimic acid from a carbon source comprising:
- a) providing a microbe selected from the group consisting of bacteria, fungi and yeast, wherein the microbe comprises one or more enzyme-encoding recombinant DNA molecules, wherein the encoded enzyme is selected from the group consisting of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and 3-dehydroshikimate dehydrogenase, and wherein the microbe further comprises an inactivating mutation in at least one DNA molecule which encodes a shikimate kinase isozyme;
- and
- b) culturing said microbe in the presence of an amount of the carbon source whereby the molar ratio of shikimic acid to quinic acid is greater than about 9.
A method for the production of shikimic acid from a carbon source comprising:
- a) providing a microbe selected from the group consisting of bacteria, fungi and yeast, wherein the microbe comprises one or more enzyme-encoding recombinant DNA molecules, wherein the enzyme is selected from the group consisting 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and 3-dehydroshikimate dehydrogenase;
- and
- b) culturing said microbe in the presence of the carbon source and a non-hydrolyzable glucose analog, whereby the molar ratio of shikimic acid to quinic acid is greater than about 9.
Dependencies are inferred from the claim text.