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US 6,613,512 B1Granted
Apparatus and method for correcting for variable velocity in microfluidic systems
AssigneeCaliper Technologies Corp.
InventorsAnne R. Kopf-Sill, Andrea W. Chow, Claudia B. Jaffe, Steven A. Sunberg, John Wallace Parce
Life of this patent
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Abstract
Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
Claims10
A method for determining the rate or extent of a reaction or assay in a microfluidic system, comprising:
- converting a first reaction or assay component having a first velocity (U1) into an assay product having a second velocity (Up) in a microfluidic channel;
- determining at least one velocity selected from the group consisting of U1 and Up;
- determining the concentration of the first reaction component [C1] or the concentration of the assay product [Cp] in a portion of the microfluidic channel;
- and
- determining the rate or extent of the reaction or assay, wherein (U1)[C1]converted=(Up)[Cp] or (U1)[C1]=(Up)[Cp].
2 more elements
Dependencies are inferred from the claim text.