Method for calibrating spectrophotometric apparatus

A method of determining the concentration of an Analyte in a Sample in a second apparatus comprising:

• (i) incorporating at least one primary calibration algorithm that uses an order of derivative of absorbance obtained for at least one of a standard set of wavelengths, on said second apparatus, where wavelengths of said standard set of wavelengths are the same as wavelengths of a wavelength calibration table on said second apparatus;
• (ii) measuring absorbance values of said sample at two or more wavelengths from the wavelength calibration table on said second apparatus;
• (iii) obtaining a derivative of said absorbance values, using said order of derivative;
• and
• (iv) calculating a concentration of said Analyte in said sample, by applying said Primary Calibration Algorithm to said derivative.

A method of determining concentration of one or more Analytes in a Sample in a second apparatus having a second linear diode array detector that contains the same number of pixels as a first linear diode array in a first apparatus, said method comprising,

• (i) incorporating at least one primary calibration algorithm from said first apparatus, that uses an order of derivative of absorbance obtained for at least one of a standard set of wavelengths, onto said second apparatus;
• (ii) measuring absorbance values of said sample at two or more wavelengths on said second apparatus;
• (iii) obtaining a derivative of said absorbance values, using said order of derivative;
• and
• (iv) calculating a concentration of said one or more Analyte in said sample, by applying said one or more Primary Calibration Algorithms to said derivative.

A method of determining the concentration of an Analyte in a Sample in a second apparatus comprising:

• (i) incorporating at least one primary calibration algorithm that uses an order of derivative of absorbance obtained for at least one of a standard set of wavelengths, on said second apparatus;
• (ii) measuring absorbance values of said sample at two or more wavelengths from said standard set of wavelengths on said second apparatus;
• (iii) obtaining a derivative of said absorbance values, using said order of derivative;
• and
• (iv) calculating a concentration of said Analyte in said sample, by applying said Primary Calibration Algorithm to said derivative.

A method of determining the concentration of an Analyte in a Sample in a second apparatus comprising:

• (i) incorporating at least one primary calibration algorithm on said second apparatus, said at least one primary calibration algorithm developed using a primary calibration set on a first apparatus, for at least one of a standard set of wavelengths;
• (ii) measuring an absorbance of said sample for two or more wavelengths from a wavelength calibration table on said second apparatus;
• (iii) determining interpolated absorbance values from said second absorbance obtained in (step (ii)), for wavelengths from said standard set of wavelengths;
• and
• (iv) calculating a concentration of said Analyte in said sample, by applying said Primary Calibration Algorithm to said interpolated absorbance.