Rice plants transformed to provide a PCK-type C4 cycle and methods of making
AssigneeJapan Tobacco Inc.
InventorsMasao Arai, Shoichi Suzuki, Nobuhiko Murai, Shigehiro Yamada, Shozo Ohta, James N. Burnell
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Abstract
The present invention relates a method for transforming a rice plant to provide it with a C4 photosynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a rice plant.
Claims12
A method for transforming a rice plant to provide the plant with a photosynthetic pathway formed in a single cell to mimic the C4 photosynthetic pathway which comprises introducing into a rice plant
- a gene coding for a phosphoenolpyruvate carboxylase (PEPC), wherein the PEPC gene is operably linked to a promoter;
- and
- a gene coding for a phosphoenolpyruvate carboxykinase (PKC), wherein the PCK gene is operably linked to a promoter and a gene coding for a chloroplast transit peptide.
A chimeric gene comprising a gene coding for a phospoenolpyruvate carboxylase (PEPC), and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been operably linked to a DNA fragment coding for a transit peptide.
A transformed rice plant comprising a gene coding for a phospoenolpyruvate carboxylase (PEPC), and a gene coding for a phosphoenolpyruvate carboxykinase (PCK), wherein the PEPC gene is operably linked to a promoter, and the PCK gene is operably linked to a promoter and a gene coding for a chloroplast transit peptide.
Dependencies are inferred from the claim text.