Cotton leaf curl virus (CLCuV) promoter and its use
AssigneeInstitute of Genetics Chinese Academy of Science
InventorsZhen Zhu, Yingqiu Xie, Yule Liu
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Abstract
The present invention is directed to a cotton leaf curl virus (CLCUV) bi-directional promoter, a method of expressing a heterologous gene in various plant tissues at a high level using the cotton leaf curl virus bi-directional promoter, and an AC2 protein factor from CLCuV genome to improve the expression level of the coat protein-coding gene promoter in the bi-directional promoter. The expression of a heterologous gene in plants is improved by using the materials and methods of the present invention.
Claims9
An isolated bi-directional promoter, comprised of the CLCuV coat protein gene promoter in one direction and the CLCuV replication protein gene promoter in the other direction, said bi-directional promoter having the nucleotide sequence of SEQ ID NO:
- 1.
An isolated promoter consisting of a fragment of the nucleotide sequence of SEQ ID NO:
- 1, wherein said fragment comprises at least bases 1-149 of SEQ ID NO:
- 1.
A method for expression of a heterologous gene in plant tissues comprising the steps of:
- (a) constructing a plant expression vector comprising the promoter of claim operably linked to a heterologous gene;
- (b) introducing the constructed plant expression vector into plant cells, and
- (c) regenerating the transformed plant cell under appropriate conditions to a plant, thereby obtaining transformed plants comprising tissues expressing said heterologous gene.
Dependencies are inferred from the claim text.