Nucleotide sequences for gene regulation and methods of use thereof

A method of expressing Factor IX in a mouse, comprising:

• a) providing:
• i) a mouse cell selected from the group consisting of an embryonic stem cell, an eight-cell embryo cell, a blastocoele cell, and a fertilized egg cell;
• ii) a nucleic acid sequence encoding Factor IX;
• iii) a promoter sequence;
• iv) one or more age regulatory sequences selected from the group consisting of SEQ ID NO:1, and SEQ ID NO:2;
• and
• v) a female host mouse;
• b) operably linking said nucleic acid sequence encoding Factor IX, said promoter sequence, and said one or more age regulatory sequences to produce a transgene;
• c) introducing said transgene into said mouse cell to create a treated cell;
• d) introducing said treated cell into said female host mouse under conditions such that said female host mouse delivers a progeny wherein said transgene is integrated into the somatic and germ cells of said progeny, and wherein said progeny expresses said Factor IX in a liver cell.

A method of expressing Factor IX in a mouse, comprising:

• a) providing:
• i) a mouse cell selected from the group consisting of embryonic stem cell, eight-cell embryo cell, blastocoele cell, and fertilized egg cell;
• ii) a nucleic acid sequence encoding Factor IX;
• iii) a promoter sequence;
• iv) an age regulatory sequence comprising SEQ ID) NO:2;
• and
• v) a female host mouse;
• b) operably linking said nucleic acid sequence encoding Factor IX, said promoter sequence, and said age regulatory sequence to produce a transgene;
• c) introducing said transgene into said mouse cell to create a treated cell;
• and
• d) introducing said treated cell into said female host mouse under conditions such that said female host mouse delivers a progeny wherein said transgene is integrated into the somatic and germ cells of said progeny, and wherein said progeny expresses said Factor IX in a liver cell.

A transgenic mouse comprising a transgene, said transgene comprising:

• i) a nucleic acid sequence encoding Factor IX;
• ii) a promoter sequence;
• and
• iii) SEQ ID NO:1;
• wherein said transgene is integrated into the somatic and germ cells of said transgenic mouse, and wherein said nucleic acid sequence encoding Factor IX is expressed in the liver of said transgenic mouse.

A transgenic mouse comprising a transgene, said transgene comprising:

• i) a nucleic acid sequence encoding Factor IX;
• ii) a promoter sequence;
• and
• iii) SEQ ID NO:2;
• wherein said transgene is integrated into the somatic and germ cells of said transgenic mouse, and wherein said nucleic acid sequence encoding Factor IX is expressed in the liver of said transgenic mouse.