Modified starch from plants, plants synthesizing the same, as well as process for their production
AssigneeBayer CropScience AG
InventorsJens Kossmann, Franziska Springer, Volker Büttcher
Life of this patent
Estimating the term…
Abstract
The present invention describes transgenic plant cells and plants which due to a reduced activity of a disproportionating enzyme (D enzyme) synthesize a modified starch. Furthermore, the starch synthesized in these plant cells and plants is described.
Claims26
A starch from a transgenic plant cell or from a plant comprising said transgenic plant cell, said plant cell exhibiting a reduced D enzyme activity as compared to a non-transformed cell and synthesizing a starch which is modified compared to starch from a non-transformed cell in its physical and/or chemical properties, the reduction of the D enzyme activity being due to
- (a) the presence of an exogenous DNA molecule the expression of which results in the inhibition of the synthesis of a functional D enzyme gene present in the cell in endogenous form;
- (b) the presence of an exogenous DNA molecule the expression of which results in the inactivation of a D enzyme gene present in the cell;
- and/or
- (c) the introduction of a mutation in a D enzyme gene present in the cell in endogenous form, with the mutation resulting in the inhibition of the expression of the gene or in the synthesis of an inactive D enzyme;
- and
- wherein said reduction of the D enzyme activity results in an increase in the phosphate content of the starch synthesized in the cell such that the starch has a phosphate content which is at least 10% higher than the phosphate content of starch from a non-transformed cell,
- wherein the exogenous DNA molecule comprises a DNA sequence that is more than 80% homologous to the DNA sequence encoding potato D enzyme deposited in Deutsche Sammlung von Mikroorganismen (DSM) having deposit number DSM 8479 or DSM 9365.
A starch from a transgenic plant cell or from a plant comprising said transgenic plant cell, said plant cell exhibiting a reduced D enzyme activity as compared to a non-transformed cell and synthesizing a starch which has a modified physical and/or chemical property compared to starch from a non-transformed cell, the reduction of the D enzyme activity being due to
- (a) the presence of an exogenous DNA molecule the expression of which results in the inhibition of the synthesis of a functional D enzyme gene present in the cell in endogenous form;
- (b) the presence of an exogenous DNA molecule the expression of which results in the inactivation of a D enzyme gene present in the cell;
- and/or
- (c) the introduction of a mutation in a D enzyme gene present in the cell in endogenous form, with the mutation resulting in the inhibition of the expression of these genes or in the synthesis of an inactive D enzyme;
- wherein the exogenous DNA molecule comprises a DNA sequence that is more than 80% homologous to the DNA sequence encoding potato D enzyme deposited in Deutsche Sammlung von Mikroorganismen (DSM) having deposit number DSM 8479 or DSM 9365;
- and
- wherein the property is selected from the group consisting of:
- (i) a higher maximum viscosity during heating in the Brabender Test;
- (ii) a higher viscosity after cooling down in the Brabender Test;
- and
- (iii) (i) and (ii).
A starch from a transgenic plant cell or from a plant comprising said transgenic plant cell, said plant cell exhibiting a reduced D enzyme activity as compared to a non-transformed cell and synthesizing a starch which as a modified physical and/or chemical property compared to starch from a non-transformed cell, the reduction of the D enzyme activity being due to
- (a) the presence of an exogenous DNA molecule the expression of which results in the inhibition of the synthesis of a functional D enzyme gene present in the cell in endogenous form;
- (b) the presence of an exogenous DNA molecule the expression of which results in the inactivation of a D enzyme gene present in the cell;
- and/or
- (c) the introduction of a mutation in a D enzyme gene present in the cell in endogenous form, with the mutation resulting in the inhibition of the expression of these genes or in the synthesis of an inactive D enzyme;
- wherein the exogenous DNA molecule comprises a DNA sequence that is more than 80% homologous to the DNA sequence encoding potato D enzyme deposited in Deutsche Sammlung von Mikroorganismen (DSM) having deposit number DSM 8479 or DSM 9365;
- and
- wherein the property is selected from the group consisting of:
- (i) an earlier rise in viscosity in the Brabender Test;
- (ii) an earlier maximum viscosity in the Brabender Test;
- and
- (iii) (i) and (ii).
A starch from a transgenic plant cell or from a plant comprising said transgenic plant cell, said plant cell exhibiting a reduced D enzyme activity as compared to a non-transformed cell and synthesizing a starch which has a modified physical and/or chemical property compared to starch from a non-transformed cell, the reduction of the D enzyme activity being due to
- (a) the presence of an exogenous DNA molecule the expression of which results in the inhibition of the synthesis of a functional D enzyme gene present in the cell in endogenous form;
- (b) the presence of an exogenous DNA molecule the expression of which results in the inactivation of a D enzyme gene present in the cell;
- and/or
- (c) the introduction of a mutation in a D enzyme gene present in the cell in endogenous form, with the mutation resulting in the inhibition of the expression of these genes or in the synthesis of an inactive D enzyme,
- wherein the exogenous DNA molecule comprises a DNA sequence that is more than 80% homologous to the DNA sequence encoding potato D enzyme deposited in Deutsche Sammlung von Mikroorganismen (DSM) having deposit number DSM 8479 or DSM 9365;
- and
- wherein the property is selected from the group consisting of:
- (i) a pastification temperature of 67.3° C.±6.7° C.;
- (ii) a maximum viscosity of 2824 BU±283 BU;
- (iii) a viscosity at the start of the holding time of 1517.3 BU±152 BU;
- (iv) a viscosity at the start of the cooling time of 641 BU±65 BU;
- and
- (v) a viscosity after cooling down of 998 BU±100 BU.
Dependencies are inferred from the claim text.