Promoter for telomerase reverse transcriptase
AssigneeGeron Corporation
InventorsGregg B. Morin, William H. Andrews
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Abstract
The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of humana diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.
Claims34
An isolated nucleic acid comprising a promoter sequence that either:
- a) is contained in lambda phage Gφ5 deposited as ATCC Accession No. 98505;
- or
- b) hybridizes to the DNA of lambda phage Gφ5 at 5 to 25° C. below the melting temperature (Tm) of a double-stranded DNA having the sequence of lambda phage Gφ5 in aqueous solution at 1 M NaCl;
- wherein the promoter sequence promotes transcription in cells endogenously expressing human telomerase reverse transcriptase (hTRT).
An isolated nucleic acid comprising a promoter sequence that is at least 80% identical to the 1.8 kilobases of SEQ. ID NO:6 that are upstream of the translation initiation site;
- wherein the promoter sequence promotes transcription in cells endogenously expressing human telomerase reverse transcriptase (hTRT).
An isolated or recombinant nucleic acid comprising a promoter sequence containing the 1.8 kB of SEQ. ID NO:6 upstream of the transcription initiation site for human telomerase reverse transcriptase (hTRT), or a fragment thereof that promotes transcription in cells endogenously expressing hTRT.
Dependencies are inferred from the claim text.