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US 6,610,828 B1Granted
Heliothis ecdysone receptor
AssigneeSyngenta Limited
InventorsIan Jepson, Alberto Martinez, Andrew James Greenland
Life of this patent
Estimating the term…
Abstract
The invention relates to an insect steroid receptor protein which is capable of acting as a gene switch which is responsive to a chemical inducer enabling external control of the gene.
Claims7
An isolated polypeptide comprising a polypeptide selected from the group consisting of:
- (a) the Heliothis virescens ecdysone steroid receptor shown in SEQ ID NO:5;
- (b) the transactivation domain of the Heliothis virescens ecdysone steroid receptor shown in amino acids 1-162 of SEQ ID NO:5;
- (c) the DNA binding domain of the Heliothis virescens ecdysone steroid receptor shown in amino acids 163-228 of SEQ ID NO:5;
- (d) the, hinge domain of the Heliothis virescens ecdysone steroid receptor shown in amino acids 229-326 of SEQ ID NO:5;
- (e) the ligand binding domain of the Heliothis virescens ecdysone receptor shown in amino acids 327-545 of SEQ ID NO:5;
- and
- (f) a derivative of the polypeptide of (a), (b), (d) or (e), wherein the derivative comprises one or more conservatively substituted amino acids and the derivative retains the function of the polypeptide, wherein the function is selected from the group consisting of ecdysone steroid receptor activity, ecdysone steroid receptor transactivation activity, ecdysone steroid receptor hinge domain activity, and ecdysone receptor ligand binding activity.
4 more elements
An isolated polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:5;
- (b) a polypeptide encoded by a nucleic acid molecule of SEQ ID NO:4;
- and
- (c) a derivative of the polypeptide of (a) or (b), wherein the derivative comprises one or more conservatively substituted amino acids and the derivative retains the function of the polypeptide, wherein the function is selected from the group consisting of ecdysone steroid receptor activity, ecdysone steroid receptor transactivation activity, ecdysone steroid receptor DNA binding activity, ecdysone steroid receptor hinge domain activity, and ecdysone receptor ligand binding activity.
1 more element
Dependencies are inferred from the claim text.