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US 6,610,822 B2Granted
Purification process
AssigneeMerck & Co., Inc.
InventorsMartin A. Chandler, Kent E. Goklen, Steven S. Lee, David J. Roush
Life of this patent
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Abstract
There is disclosed a process for purifying a natural product using a two-phase, multi-solvent system followed by vacuum concentration and back extraction. The method allows for the removal of impurities by controlling the polarity balance of a two-phase system by manipulating the proportions of the four solvents and subsequently the relative distribution of the product versus the impurities.
Claims16
A process for purifying an echinocandin or a pneumocandin comprising
- a) extraction of a crude product comprising echinocandin or pneumocandin with a suitable solvent;
- b) vacuum concentration of the extract containing the product and solvent;
- c) partitioning of the solvent into a second solvent extract;
- d) washing of the second solvent extract;
- e) vacuum concentration of the washed second solvent extract;
- f) back extracting with the first solvent;
- g) vacuum concentration of the extract containing the product and the first solvent;
- and
- h) precipitation of the product from step g).
6 more elements
A process for purifying Compound I of the formula
- comprising
- a) extraction of a crude product of Compound I with a suitable solvent;
- b) vacuum concentration of the extract containing the product and solvent;
- c) partitioning of the solvent into a second solvent extract;
- d) washing of the second solvent extract;
- e) vacuum concentration of the washed second solvent extract;
- f) back extracting with the first solvent;
- g) vacuum concentration of the extract containing the product and the first solvent;
- and
- h) precipitation of the product from step g).
7 more elements
Dependencies are inferred from the claim text.