Protection of plants against viral infection
AssigneesMonsanto Technology LLC, Washington University
InventorsRoger N. Beachy, Robert T. Fraley, Stephen G. Rogers
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Abstract
The invention involves recombinant, double-stranded DNA that contains a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus, a DNA sequence that causes the production of an RNA sequence encoding the coat protein of said plant virus, and a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence; which double-stranded DNA can be used in a method for genetically transforming plants to produce genetically transformed plant cells and plants that are resistant to virus infection.
Claims40
A method of producing genetically transformed plant cells which are resistant to infection by a plant virus, comprising the steps of:
- (a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising
- (i) a promoter which functions in plant cells to cause the production of RNA sequences of said plant virus;
- (ii) a DNA sequence that causes the production of an RNA sequence, said RNA sequence encoding the coat protein of said plant virus;
- and
- (iii) a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence;
- and
- recovering transformed plant cells which have increased resistance to infection by said plant virus.
A recombinant, double-stranded DNA molecule comprising in sequence:
- (a) a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus;
- (b) a DNA sequence that causes the production of an RNA sequence, said RNA sequence encoding the coat protein of said plant virus;
- and
- (c) a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence,
- said molecule being incapable of causing systemic infection in plants.
A transformed plant cell consisting chromosomal DNA comprised of:
- (a) a promoter which functions in, plant cells to cause the production of RNA sequences of a plant virus;
- (b) a DNA sequence that causes the production of an RNA sequence, said RNA sequence encoding the coat protein of said plant virus;
- and
- (c) a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence.
A recombinant, double-stranded DNA molecule comprising in sequence:
- (a) a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus;
- (b) a DNA sequence that causes the production in an RNA sequence, said RNA sequence encoding the coat protein of said plant virus;
- and
- (c) a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence,
- said promoter being from a source other than cauliflower mosaic virus.
A virus-resistant dicotyledonous plant comprising in its chromosomal genome a DNA construct comprising a viral coat protein structural gene downstream from a plant-expressible promoter.
A method for producing a virus-resistant dicotyledonous plant, comprising the steps of:
- (a) introducing into the chromosomal genome of a dicotyledonous plant cell a recombinant DNA molecule comprising a viral coat protein structural gene downstream from a plant-expressible promoter;
- (b) recovering transformed plant cells;
- and
- (c) regenerating virus-resistant dicotyledonous plants from said transformed cells.
Dependencies are inferred from the claim text.