Sunflower disease resistance genes
AssigneePioneer Hi-Bred International, Inc.
InventorsDennis L. Bidney, Xu Hu, Guihua Lu
Life of this patent
Estimating the term…
Abstract
Compositions and methods for enhancing disease resistance in plants are provided. Compositions comprise sunflower resistance gene analogs. The methods involve transforming a plant with a resistance nucleotide sequence. The methods find use in enhancing broad-based resistance in plants to pathogens. Also provided are transformed plants, plant cells, tissues, and seeds having enhanced disease resistance. The R gene analogs can be used as molecular markers for disease resistance.
Claims40
A method for creating or enhancing disease resistance in a plant, said method comprising:
- (a) introducing into a plant cell a recombinant expression cassette comprising a sunflower R nucleotide sequence operably linked to a promoter that drives expression in a plant, wherein said R nucleotide sequence is selected from the group consisting of:
- (i) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (ii) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) culturing the plant cell under plant cell growing conditions;
- and
- (c) regenerating from the plant cell a whole plant, wherein the plant has enhanced or newly created disease resistance.
A plant having stably incorporated in its genome a DNA construct, said construct comprising a promoter that drives expression in a plant operably linked to a sunflower R nucleotide sequence, wherein said R nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (b) the nucleotide sequence set forth in SEQ ID NO:
- 1.
A plant cell having stably incorporated in its genome a DNA construct, said construct comprising a promoter that drives expression in a plant cell operably linked to a sunflower R nucleotide sequence, wherein said R nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (b) the nucleotide sequence set forth in SEQ ID NO:
- 1.
An isolated nucleotide sequence said sequence comprising at least one of the nucleotide sequences selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (b) the nucleotide sequence set forth in SEQ ID NO:
- 1.
A method for modulating cellular metabolism in a plant, said method comprising
- (a) introducing into a plant cell a recombinant expression cassette comprising a promoter that drives expression in a plant cell operably linked to a nucleic acid sequence selected from the group consisting of;
- (i) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (ii) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) culturing the plant cell under plant cell growing conditions;
- and
- (c) regenerating from the plant cell a whole plant, wherein the plant has modulated cellular metabolism.
A method for creating or enhancing disease resistance in a plant, said method comprising:
- (a) introducing into a plant cell a recombinant expression cassette comprising a sunflower R nucleotide sequence operably linked to a promoter that drives expression in a plant, wherein said R nucleotide sequence comprises a nucleotide sequence that has at least about 95% sequence identity to the sequence set forth in SEQ ID NO:
- 1, wherein said nucleotide sequence encodes an R protein;
- (b) culturing the plant cell under plant cell growing conditions;
- and
- (c) regenerating from the plant cell a whole plant, wherein the plant has enhanced or newly created disease resistance.
A plant having stably incorporated in its genome a DNA construct, said construct comprising a promoter that drives expression in a plant operably linked to a sunflower R nucleotide sequence, wherein said R nucleotide sequence comprises a nucleotide sequence that has at least about 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes an R protein.
A plant cell having stably incorporated in its genome a DNA construct, said construct comprising a promoter that drives expression in a plant cell operably linked to a sunflower R nucleotide sequence, wherein said R nucleotide sequence comprises a nucleotide sequence that has at least about 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes an R protein.
An isolated nucleotide sequence, said sequence comprising a nucleotide sequence that has at least about 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes an R protein.
A method for modulating cellular metabolism in a plant, said method comprising
- (a) introducing into a plant cell a recombinant expression cassette comprising a promoter that drives expression in a plant cell operably linked to a sunflower R nucleotide sequence, wherein said R nucleotide sequence comprises a nucleotide sequence that has at least about 95% sequence identity to the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence encodes an R protein;
- (b) culturing the plant cell under plant cell growing conditions;
- and
- (c) regenerating from the plant cell a whole plant, wherein the plant has modulated cellular metabolism.
Dependencies are inferred from the claim text.