Cholesterol lowering structured lipids containing omega 3 polyunsaturated fatty acids and their process thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsReena Rao, Kari Sambaiah, Belur Ramaswamy Lokesh
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Abstract
Unique structured lipids obtained from interestifying coconut oil with free fatty acids obtained from hydrolysis of triglycerides of an animal source, said structured lipids rich in omega-3-poly unsaturated fatty acid and medium chain fatty acid and a process for the production of said structured lipids.
Claims19
A process for production of cholesterol lowering structured lipids from cod liver oil rich in omega 3 polyunsaturated fatty acids (omega 3 PUFA), said process comprising:
- (a) hydrolyzing triglycerides of said animal source by a known method, to obtain free fatty acids rich in omega 3 PUFA;
- (b) interesterifying coconut oil with the free fatty acids obtained from the step (a) at preferable molar ratio of 1:5;
- (c) incubating with immobilized lipase at a temperature range of 37-55° C. for a period of 6-48 hours using a hydrocarbon solvent for enzymatic acidolysis thereby incorporating the required acyl groups into the specific positions of the triacylglycerols;
- (d) separating the reaction products by adsorption chromatography using one or more organic solvents selected from ethers, hexane and optionally with 1 part of acetic acid to obtain structured lipids;
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- (e) recovering the structured lipids by scaling up in the range of 85-90%.
Unique structured lipids obtained from interestifying coconut oil with free fatty acids obtained from hydrolysis of triglycerides of an animal source, said structured lipids contain up to 14 mol % omega-3-poly unsaturated fatty acid and rich in medium chain fatty acid.
Dependencies are inferred from the claim text.