Fluorescence-labeled probe for DNA and a fluorescence-labeled plasmid
AssigneeTokyo Institute of Technology
InventorsToshinori Sato, Yoshio Okahata
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Abstract
This invention provides a precursor compound of a novel fluorescence-labeled probe for DNA, prepared by covalent binding of a fluorescent substance such as fluorescein isothiocyanate, wherein a conjugating group was bounded, with aminoalkylate phenylamine. This invention also provides a novel fluorescence-labeled probe for DNA by diazotizing amino group of phenylamine contained in the precursor compound. Furthermore, a fluorescence labeled plasmid can be produced by conjugating the fluorescence-labeled probe and purine ring of guanine contained in the DNA plasmid through diazonium group.
Claims12
A precursor compound of a fluorescence-labeled probe for a DNA, comprising:
- a residue of a fluorescence substance containing a hydrogen atom wherein said hydrogen atom is removed from said fluorescence substance;
- a conjugating group that binds to said residue;
- an alkylamino group having an alkyl group and amino group, said amino group being bonded to said conjugating group and said alkyl group being a linear or branched alkyl group having 1 to 16 carbon atoms;
- and
- a phenylamine having phenyl group and amino group, said alkyl group being bonded to said phenyl group at the para-position of said phenyl group, and said amino group being a primary amino group.
A fluorescence-labeled probe for a DNA, comprising:
- a residue of a fluorescence substance containing a hydrogen atom wherein said hydrogen atom is removed from said fluorescence substance;
- a conjugating group that binds to said residue;
- an alkylamino group having an alkyl group and amino group, said amino group being bonded to said conjugating group and said alkyl group being a linear or branched alkyl group having 1 to 16 carbon atoms;
- and
- a phenyldiazonium group having phenyl group and diazonium group, said alkyl group being bonded to said phenyl group at the para-position of said phenyl group, and wherein said phenyl group is not substituted by a halogen atom.
A fluorescence-labeled plasmid, comprising:
- a residue of a fluorescence substance containing a hydrogen atom wherein said hydrogen atom is removed from said fluorescence substance;
- a conjugating group that binds to said residue;
- an alkylamino group having an alkyl group and amino group, said amino group being bonded to said conjugating group and said alkyl group being a linear or branched alkyl group having 1 to 16 carbon atoms;
- a phenyldiazonium group having phenyl group and diazonium group, said alkyl group being bonded to said phenyl group at the para-position of said phenyl group, and wherein said phenyl group is not substituted by a halogen atom;
- and
- a plasmid composed of a DNA containing guanine, wherein said diazonium group binds to said plasmid through purine ring of said guanine.
Dependencies are inferred from the claim text.