Polypeptides having branching enzyme activity and nucleic acids encoding same
AssigneeNovozymes A/S
InventorMari L. Shinohara
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having branching enzyme activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Claims18
An isolated polypeptide having branching enzyme activity selected from the group consisting of:
- a) a polypeptide having an amino acid sequence which has at least 80% homology with amino acids 1 to 620 of the amino acid sequence of SEQ ID NO:2;
- b) a polypeptide having an amino acid sequence which has at least 80% homology with the branching enzyme amino acid sequence encoded by the nucleic acid sequence contained in plasmid pT7Blue contained in E. coli DH12S, DSM 12607;
- and
- c) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under hybridization conditions comprising prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, followed by washing three times for 15 minutes using 2×SSC, 0.2% SDS at 60° C. with (i) nucleotides 4 to 1863 of the nucleic acid sequence of SEQ ID NO:1, (ii) nucleotides 1 to 1863 of SEQ ID NO:1, or (iii) a full complementary strand of (i), or (ii).
An isolated polypeptide having branching enzyme activity comprising a polypeptide having an amino acid sequence which has at least 80% homology with amino acids 1 to 620 of the amino acid sequence of SEQ ID NO:2.
An isolated polypeptide having branching enzyme activity comprising a polypeptide having an amino acid sequence which has at least 80% homology with the branching enzyme amino acid sequence encoded by the nucleic acid sequence contained in plasmid pT7Blue contained in E. coli DH12S, DSM 12607.
An isolated polypeptide having branching enzyme activity comprising a polypeptide which is encoded by a nucleic acid sequence which hybridizes under hybridization conditions comprising prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μ/ml sheared and denatured salmon sperm DNA, and 35% formamide, followed by washing three times for 15 minutes using 2×SSC, 0.2% SDS at 60° C. with nucleotides 4 to 1863 of the nucleic acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.