Vertebrate embryonic pattern-inducing proteins and uses related thereto
OwnerPhilip W. Ingham, Andrew P. McMahon +1inventors
InventorsPhilip W. Ingham, Andrew P. McMahon, Clifford J. Tabin
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Abstract
The present invention concerns the discovery that proteins encoded by a family of vertebrate genes, termed here hedgehog-related genes, comprise morphogenic signals produced by embryonic patterning centers, and are involved in the formation of ordered spatial arrangements of differentiated tissues in vertebrates. The present invention makes available compositions and methods that can be utilized, for example to generate and/or maintain an array of different vertebrate tissue both in vitro and in vivo.
Claims36
An isolated nucleic acid encoding a polypeptide comprising a hedgehog amino acid sequence at least 80% identical to an amino acid sequence selected from SEQ ID No:
- 8, SEQ ID No:
- 11, SEQ ID No:
- 12, or SEQ ID No:
- 13, or a fragment of said hedgehog amino acid sequence, wherein said hedgehog amino acid sequence, or fragment thereof, binds to a naturally occurring patched receptor.
An isolated nucleic acid encoding a polypeptide, which polypeptide comprises a hedgehog amino acid sequence or a fragment thereof, which sequence or fragment comprises at least 150 contiguous amino acid residues from the N-terminal half of a polypeptide selected from SEQ ID No:
- 8, SEQ ID No:
- 11, SEQ ID No:
- 12, or SEQ ID No:
- 13, wherein said hedgehog amino acid sequence or fragment thereof binds to a naturally occurring patched receptor.
A nucleic acid comprising:
- (A) a coding sequence for a polypeptide comprising a hedgehog amino acid sequence at least 80% identical to an amino acid sequence selected from SEQ ID No:
- 8, SEQ ID No:
- 11, SEQ ID No:
- 12, or SEQ ID No:
- 13, which amino acid sequence binds to a naturally occurring patched receptor;
- and
- (B) a transcriptional regulatory sequence operably linked to said coding sequence and heterologous thereto.
Dependencies are inferred from the claim text.