Loading patent…
AssigneeBASF SE
InventorsMark Henry Pausch, Bradley Alton Ozenberger, John Richard Hadcock, Laura Alicia Price, Eileen Marie Kajkowski, Donald Richard Kirsch, Deborah Tardy Chaleff
Estimating the term…
A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein a subunit or portions thereof fused to DNA sequences from the yeast G protein in a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.
A transformed yeast cell comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous G protein coupled receptor gene, each said gene being under the control of a separate promoter, and a gene mutation causing increased sensitivity to receptor activation selected from the group consisting of sst2, STE50, sgvl, ste2, ste3, pik1, afr1, msg5, and sig1, wherein said heterologous G protein-coupled receptor gene does not include a coding sequence from a yeast G protein-coupled receptor gene fused thereto.
A transformed yeast cell comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous G protein-coupled receptor gene, each said gene being under the control of a separate promoter, and a mutation at a gene that permits transcriptional activation of pheromone-responsive genes without cell cycle arrest, wherein said heterologous G protein-coupled receptor gene does not include a coding sequence from a yeast G protein-coupled receptor gene fused thereto.
Dependencies are inferred from the claim text.