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US 6,607,901 B1Granted
Alkaline alpha-galactosidase
AssigneeState of Israel Ministry of Agriculture Volcani Research Center
InventorsArthur Schaffer, Gao Zhifang
Life of this patent
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Abstract
An enzyme isolated from an organism that metabolizes alpha-galactosyl containing saccharides, comprising an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galatohydrolase) with optimal activity in the neutral to alkaline pH range, and which hydrolyzes a variety of alpha-galactose containing saccharides, in particular raffinose. The enzyme is preferably a protein monomer and an ex-alpha-galactosidase.
Claims18
An isolated enzyme from a plant of the Cucurbit family comprising,
- a) an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galactohydrolase) activity;
- b) a Km for the substrates raffinose of less than about 10 mM;
- c) optimal activity in the range of pH 7.0 to pH 8.0;
- d) molecular mass of about 84 kDa, as determined by gel electrophoresis;
- the enzyme being non-glycosylated.
2 more elements
An isolated plant enzyme having,
- a) an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galactohydrolase) activity;
- b) a Km for the substrates raffinose of less than about 10 mM;
- c) optimal activity in the range of pH 7.0 to pH 8.0;
- d) molecular mass of about 84 kDa, as determined by gel electrophoresis;
- the isolated plant enzyme being non-glycosylated and having an N-terminal sequence as set forth in SEQ. ID. NO:
- 3.
3 more elements
Dependencies are inferred from the claim text.