Method for detection of hTR and hTERT telomerase-associated RNA in plasma or serum
AssigneeOncoMEDx, Inc.
InventorsMichael S. Kopreski, Christopher D. Gocke
Life of this patent
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Abstract
This invention relates to methods of detecting extracellular hTR RNA and hTERT RNA in blood, plasma, serum, and other bodily fluids. The invention thereby provides an aid for the detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease.
Claims30
A method for detecting extracellular human telomerase RNA template (hTR) RNA in blood plasma or serum for detecting, diagnosing, monitoring, treating or evaluating neoplastic disease comprising cells that express hTR, the method comprising the steps of:
- a) extracting mammalian, heterogeneous extracellular RNA from human blood plasma or serum;
- b) amplifying a portion of the extracted extracellular RNA or cDNA produced therefrom wherein said portion comprises hTR RNA to produce an amplified product, and wherein amplification is performed qualitatively or quantitatively using primers or probes that target hTR RNA or the corresponding cDNA;
- and
- c) detecting qualitatively or quantitatively the amplified product produced from hTR RNA or corresponding cDNA.
A method of detecting extracellular human telomerase RNA template (hTR) RNA in a bodily fluid for detecting, diagnosing, monitoring, treating or evaluating neoplastic disease comprising cells that express hTR, the method comprising the steps of:
- a) extracting mammalian, heterogeneous extracellular RNA from a human bodily fluid;
- b) amplifying a portion of the extracted, extracellular RNA or cDNA produced therefrom wherein said portion comprises hTR RNA to produce an amplified product, and wherein amplification is performed qualitatively or quantitatively using primers or probes that target hTR RNA or the corresponding cDNA;
- and
- c) detecting qualitatively or quantitatively the amplified product produced from hTR RNA or corresponding cDNA.
A method for selecting a human with a cancer for a cancer therapy, the method comprising the steps of detecting human telomerase RNA in an acellular fraction of a hTR in said acellular fraction of said bodily fluid for the cancer therapy.
A method of identifying a human having human telomerase RNA template (hTR) expressing cells or tissue, the method comprising the steps of:
- a) extracting mammalian, heterogeneous extracellular RNA from blood plasma or serum from the human;
- b) amplifying a portion of the extracted extracellular RNA or cDNA produced therefrom wherein said portion comprises extracellular hTR RNA to produce an amplified product, and wherein amplification is performed qualitatively or quantitatively using primers or probes that target hTR RNA or the corresponding cDNA;
- and
- c) detecting the amplified product produced from hTR RNA or corresponding cDNA.
A method of selecting a human with cancer for a cancer therapy, the method comprises the steps of:
- a) extracting mammalian, heterogeneous extracellular RNA from the human's blood plasma or serum;
- b) amplifying a portion of the extracted extracellular RNA or cDNA produced therefrom wherein said portion comprises human telomerase RNA template (hTR) RNA to produce an amplified product, and wherein amplification is performed qualitatively or quantitatively using primers or probes that target hTR RNA or the corresponding cDNA;
- c) detecting the amplified product produced from hTR RNA or corresponding cDNA, and
- d) selecting the human with cancer for the cancer therapy when the amplified product is detected.
A method for detecting human telomerase RNA template (hTR), or cDNA reverse-transcribed therefrom, comprising the steps of extracting RNA comprising human telomerase RNA template (hTR) RNA from blood plasma or serum, with or without converting said RNA to cDNA, hybridizing said RNA or cDNA to a detectably-labeled probe specific for hTR RNA or cDNA, and detecting hybridization of hTR RNA or cDNA with the detectably-labeled probe.
A method for detecting human telomerase RNA template (hTR) RNA, or cDNA reverse-transcribed therefrom, comprising the steps of extracting RNA comprising hTR RNA from an acellular fraction of a bodily fluid, with or without converting said RNA to cDNA, hybridizing said RNA or cDNA to a detectably-labeled probe specific for hTR RNA or cDNA, and detecting hybridization of hTR RNA or cDNA with the detectably-labeled probe.
Dependencies are inferred from the claim text.