Diacylglycerol acyltransferase (DGAT) assay
AssigneeWarner-Lambert Company
InventorsRandy Ranjee Ramharack, Mark Allan Spahr
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for measuring diacylglycerol acetyltransferase (DGAT) activity which utilizes a novel solvent system to reduce and/or eliminate the activities of related compounds. The present invention also discloses a method for determining whether a compound is useful for modulating DGAT biological activity. The method is capable of being utilized for mass screening of compounds as modulators of the biological activity of DGAT.
Claims48
A method for measuring diacylglycerol acetyltransferase (DGAT) activity, said method comprising the steps of:
- (a) providing at least one DGAT substrate;
- (b) combining microsomes having DGAT associated therewith with the DGAT substrate to form a reaction mixture;
- (c) incubating the mixture of the microsomes and the DGAT substrate for a predetermined time interval;
- (d) terminating the reaction of DGAT with the DGAT substrate;
- and
- (e) detecting triglyceride production as an indicator of DGAT biological activity.
A method for determining whether a compound is useful for modulating diacylglycerol acyltransferase (DGAT) biological activity, said method comprising the steps of:
- (a) providing DGAT substrates;
- (b) providing microsomes having DGAT associated therewith;
- (c) contacting a compound to be analyzed for its ability to modulate DGAT biological activity with the substituents set forth in (a) and (b) to form a reaction mixture;
- (d) incubating the reaction mixture for a predetermined time interval;
- (e) terminating any reaction of DGAT with the at least one DGAT substrate in the reaction mixture;
- and
- (f) measuring DGAT biological activity, wherein a change in DGAT biological activity, relative to a control not contacted with the compound, indicates that the compound modulates DGAT biological activity.
Dependencies are inferred from the claim text.