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US 6,607,891 B1Granted
Method of assaying insulin-like growth factor
AssigneeMitsubishi Chemical Corporation
InventorMakoto Takada
Life of this patent
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Abstract
A pretreatment method for assaying a substance which comprises mixing a biological specimen with at least one pretreating agent selected from among surfactants and alkali agents, thus releasing binding proteins in the biological specimen from the substance to be assayed and, at the same time, inactivating the proteins by irreversible denaturation to thereby eliminate the effects of the binding proteins coexisting in the biological specimen.
Claims11
A method of assaying an insulin-like growth factor (IGF), consisting essentially of:
- (i) adding at least one pretreating agent selected from the group consisting of an anionic surfactant and an alkali agent to a biological sample, at such a level to not negatively affect any activity of any IGF in said biological sample in an IGF determination assay and to dissociate any complex of IGF and IGF binding protein in said biological sample and to inactivate any IGF binding protein in said biological sample, to obtain a mixture of said biological sample and said pretreating agent;
- and
- (ii) subjecting said mixture of said biological sample and said pretreating agent to an assay for IGF presence or amount.
Dependencies are inferred from the claim text.