Methods of detecting growth differentiation factor-8
AssigneeThe Johns Hopkins University School of Medicine
InventorsSe-Jin Lee, Alexandra C. McPherron
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Abstract
The present invention relates to a method for detecting a polynucleotide encoding GDF-8 in a sample by contacting the sample with an oligonucleotide probe that hybridizes specifically with a polynucleotide encoding GDF-8; and detecting specific hybridization of the oligonucleotide probe to a polynucleotide in the sample, thereby detecting a polynucleotide encoding GDF-8 in the sample. The sample can be a tissue sample or a cell sample, for example, a muscle cell sample, which can be obtained, for example, from a mammal such as a bovine, ovine or porcine mammal, or a human.
Claims33
A method for detecting a polynucleotide encoding growth differentiation factor-8 (GDF-8) in a sample, the method comprising:
- a) contacting the sample with an oligonucleotide probe that hybridizes specifically with a polynucleotide encoding GDF-8,
- wherein the oligonucleotide probe comprises at least about a 15 nucleotide portion of a nucleic acid molecule encoding a mammalian or avian GDF-8;
- and
- b) detecting specific hybridization of the oligonucleotide probe to a polynucleotide in the sample, wherein said specific hybridization is stable to washing in 2×SSC at 65° C., thereby detecting a polynucleotide encoding GDF-8 in the sample.
A method for detecting a polynucleotide encoding growth differentiation factor-8 (GDF-8) in a sample, the method comprising:
- a) contacting the sample with an oligonucleotide probe that hybridizes specifically with a polynucleotide encoding GDF-8,
- wherein the oligonucleotide probe comprises at least about a 15 nucleotide portion of a nucleic acid molecule encoding a mammalian or avian GDF-8, and
- wherein the oligonucleotide probe comprises a primer of a polymerase chain reaction (PCR) primer pair comprising a forward primer and a reverse primer;
- and
- b) detecting specific hybridization of the oligonucleotide probe to a polynucleotide in the sample by performing PCR for 40 cycles at 94° C. for 2 minutes, 60° C. for 1 minute, and 72° C. for 2 minutes, thereby detecting a polynucleotide encoding GDF-8 in the sample.
Dependencies are inferred from the claim text.