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US 6,607,883 B1Granted
Polymerase chimeras
AssigneeRoche Diagnostics GmbH
InventorsBruno Frey, Britta Villbrandt, Dietmar Schomburg, Harald Sobek, Waltraud Ankenbauer
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AssigneeRoche Diagnostics GmbH
InventorsBruno Frey, Britta Villbrandt, Dietmar Schomburg, Harald Sobek, Waltraud Ankenbauer
Estimating the term…
The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.
A polymerase chimera comprising functional amino acid fragments of at least two different polymerases, wherein the functional amino acid fragments are active in the polymerase chimera, a domain having polymerase activity is derived from the first polymerase and a domain havine 3′-5′ exonuclease activity is derived from the second polymerase, and wherein the amino acid sequence of the polymerase chimera is SEQ ID NO:
A nucleic acid that encodes a polymerase chimera comprising the sequence of SEQ ID NO. 2.
Dependencies are inferred from the claim text.