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US 6,607,882 B1Granted
Regulation of endogenous gene expression in cells using zinc finger proteins
AssigneeSangamo BioSciences, Inc.
InventorsGeorge N. Cox, III, Casey C. Case, Stephen P. Eisenberg, Eric E. Jarvis, Sharon K. Spratt
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Abstract
The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
Claims32
A method of activating expression of a developmentally silenced endogenous cellular gene in a cell;
- the method comprising the steps of:
- (a) administering a nucleic acid to the cell, wherein the nucleic acid comprises a sequence encoding a first zinc finger protein operably linked to a promoter, wherein the first zinc finger protein is engineered;
- and
- (b) contacting a first target site in the endogenous cellular gene with the first zinc finger protein, wherein the Kd of the zinc finger protein is less than about 25 nM;
- wherein said contacting results in activation of expression of the endogenous cellular gene to at least about 150%.
2 more elements
A method of activating expression of a developmentally silenced endogenous cellular gene in a cell the method comprising the steps of:
- (a) administering a nucleic acid to the cell, wherein the nucleic acid comprises a sequence encoding a fusion zinc finger protein operably linked to a promoter, wherein the fusion zinc finger protein comprises six fingers and a regulatory domain;
- and
- (b) contacting a first target site in the endogenous cellular gene with the fusion zinc finger protein, wherein the Kd of the zinc finger protein is less than about 25 nM;
- thereby activating expression of the endogenous cellular gene to at least about 150%.
1 more element
Dependencies are inferred from the claim text.