Mirror-symmetrical selection and evolution of nucleic acids
OwnerJens Peter Furste, Rolf Bald +1inventors
InventorsJens Peter Furste, Rolf Bald, Volker A. Erdmann
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Abstract
A method is disclosed for identifying and producing L-nucleic acids which interact with a target molecule having a natural configuration, as well as the L-nucleic acids produced by this method. Also disclosed is the use of D-nucleic acids which bind to the optical antipode of the target molecule as a matrix for producing L-nucleic acids with an identical sequence, and pharmaceutical compositions and kits which contain the disclosed L-nucleic acids.
Claims40
Method of producing L-nucleic acids binding to a target molecule having natural configuration, said method comprising the following steps:
- (a) producing a heterogeneous population of D-nucleic acids;
- (b) bringing the population of step (a) into contact with a racemic mixture of the target molecule;
- (c) separating the D-nucleic acids not binding to the racemic mixture of the target molecule;
- (d) sequencing the D-nucleic acids binding to the racemic mixture of the target molecule;
- and
- (e) producing L-nucleic acids, the sequence of which is identical to the sequences of the D-nucleic acids determined in step (d).
Method of producing L-nucleic acids comprising the following steps:
- (a) producing a heterogeneous population of L-nucleic acids;
- (b) bringing the population of step (a) into contact with a target molecule;
- (c) separating the L-nucleic acids not binding to the target molecule;
- (d) sequencing the L-nucleic acids binding to the target molecule;
- (e) producing L-nucleic acids, the sequence of which is identical to the sequences determined in step (d).
Dependencies are inferred from the claim text.