Gene transcription and ionizing radiation: methods and compositions
AssigneeArch Development Corporation
InventorsRalph R. Weichselbaum, Dennis E. Hallahan, Vikas P. Sukhatme, Donald W. Kufe
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Abstract
The present invention provides a DNA molecule comprising a radiation responsive enhancer-promoter operatively linked to an encoding region that encodes at least one polypeptide. An encoding region can comprise a single encoding sequence for a polypeptide or two or more encoding sequences encoding DNA binding, activation or repression domains of a transcription factor. Processes for regulating polypeptide expression and inhibiting tumor growth using such DNA molecules are also provided.
Claims35
An isolated and purified DNA molecule comprising a radiation inducible promoter operatively linked to an encoding region that encodes at least one polypeptide, which inhibits the growth of a cell, and that one desires to have expressed in a radiation inducible manner, wherein said radiation inducible promoter comprises a c-jun promoter or at least one CArG domain of an Egr-1 promoter.
A cell transformed or transfected with a DNA molecule comprising a radiation inducible promoter operatively linked to an encoding region that encodes at least one polypeptide, which inhibits the growth of the cell, wherein the radiation inducible promoter comprises a c-jun promoter or at least one CArG domain of an Egr-1 promoter.
A vehicle containing a DNA molecule comprising a radiation inducible promoter operatively linked to an encoding region that encodes at least one polypeptide, which polypeptide inhibits the growth of a cell, and that one desires to have expressed in a radiation inducible manner, wherein said radiation inducible promoter comprises a c-jun promoter or at least one CArG domain of an Egr-1 promoter.
A vehicle containing a DNA molecule comprising a radiation inducible promoter operatively linked to an encoding region that encodes tumor necrosis factor-alpha, wherein the radiation inducible promoter comprises six CArG domains of an Egr-1 promoter.
Dependencies are inferred from the claim text.