Method and medium for in vitro culture of human embryos
AssigneesLuminis Pty. Ltd., Fertilitescentrum AB
InventorsSarah Robertson, Matts F. Wikland, Cecilia Sjoblom
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Abstract
Disclosed is a medium for the propagation of early stage embryos to blastocyst stage. The medium contains an effective amount of GM-CSF to increase the percentage of pre-blastocyst embryos which develop to transfer ready blastocysts. Also disclosed is a method of growing early stage human embryos to transfer ready blastocysts. The method includes the step of incubating the embryos in vitro in a culture medium containing an effective amount of human GM-CSF for a time and under conditions to increase the proportion of transfer ready blastocysts. An IVF program that includes the method of growing early stage human embryos to transfer ready blastocysts is also disclosed.
Claims20
A method of growing preblastocyst human embryos, the method including the step of incubating the embryos in vitro in a culture medium containing an effective amount of human GM-CSF to increase the chance of implantation of the embryos, the amount of GM-CSF being sufficient to increase the proportion of blastocysts formed from preblastocyst embryos when compared to embryos incubated in a medium lacking GM-CSF.
An IVF program comprising the steps of:
- contacting a human egg with a human sperm to form a conceptus
- growing the resulting conceptus in vitro in a chemically defined medium containing an effective amount of human GM-CSF to increase the chance of implantation of the embryos
- transferring the embryo into a compatible human uterus,
- wherein the amount of human GM-CSF is sufficient to increase the proportion of blastocysts formed from preblastocyst embryos when compared to culturing the resulting conceptus in a medium lacking GM-CSF.
Dependencies are inferred from the claim text.