Protease variants and compositions
AssigneeNovozymes A/S
InventorsPeter Kamp Hansen, Peter Bauditz, Frank Mikkelsen
Life of this patent
Estimating the term…
Abstract
A protease subtilase enzyme, characterized by an insertion in at least one active site loop. The enzymes exhibit improved wash performance in a detergent in comparison to its parent enzyme if it is a subtilase variant.
Claims77
A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation comprises at least one of the following:
- (a) an insertion of at least one amino acid in the active site loop located at positions 125-132;
- (b) an insertion of at least one amino acid in the active site loop located at positions 181-195;
- and
- (c) an insertion of at least one amino acid in the active site loop located at positions 202-204;
- wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.
A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation comprises at least one of the following:
- (a) an insertion of at least one amino acid in the active site loop located at positions 33-35 and 37-43;
- or
- (b) an insertion of at least one amino acid in the active site loop located at positions 153-173;
- wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.
A modified subtilase comprising a mutation in an amino acid sequence of a subtilase, wherein the mutation comprises at least one of the following:
- (a) an insertion of at least two amino acids in the active site loop located at positions 95-103;
- or
- (b) an insertion of at least two amino acids in the active site loop located at positions 218-219;
- wherein the positions are numbered according to the amino acid sequence of the mature subtilisin BPN′.
The modified subtilase of 51, wherein the insertion comprises at least A, G, S, or T.
Dependencies are inferred from the claim text.