Influenza vaccine
AssigneeVlaams Interuniversitair Instituut Voor Biotechnologie
InventorsWalter Charles Fiers, Tom Maria Deroo, Willy Alfons Min Jou
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Abstract
A recombinant neuraminidase obtainable by culturing in a suitable culture medium host cells which are transformed with a neuraminidase expression vector or infected with a virus which is transformed with a neuraminidase expression vector, wherein the expression vector comprises at least a part of the coding region of a neuraminidase gene of an influenze virus minus the region which codes for the membrane anchor, or a modified version thereof, preceded in phase by a signal sequence; and isolating the expression product neuraminidase from the culture medium. The invention further relates to a vaccine in which the recombinant neuraminidase is applied.
Claims9
Recombinant neuraminidase free of hemagglutinin, influenza viral RNA or other influenza virus components, obtainable by:
- a. culturing in a suitable culture medium host cells which are transformed with a neuraminidase expression vector or infected with a virus which is transformed with a neuraminidase expression vector, wherein the expression vector or virus comprises at least part of the coding region of the neuraminidase, having antigenic properties corresponding to naturally occurring neuraminidase, preceded in phase by a type I signal sequence, wherein the expression vector or virus does not include in phase with and immediately preceding the neuraminidase coding region a sequence coding for at least the membrane anchor region of the neuraminidase so that the membrane anchor function is not retained;
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- b. isolating the expression product neuraminidase from the culture medium.
Recombinant influenza neuraminidase homogeneously purified judging from the silver staining of SDS/PAGE gels, obtainable by culturing in a suitable culture medium host cells transformed with the recombinant expression vector pPP1VNAfls (LMBP 3223) and isolating the expression product neuraminidase from the culture medium.
Dependencies are inferred from the claim text.