Method and means for sorting and identifying biological information
OwnerGeorge Pieczenikinventor
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Abstract
In one aspect the invention discloses a matrix comprising a discrete population of random oligopeptides of the same length, the length being selected from about 4 to about 12 L-amino acid residues, the population comprising at least 10% of all amino acid sequences of the selected length; and a heterogeneous population of antibodies comprising antibodies capable of binding to substantially every member of the oligopeptide population.
Claims23
A population of recombinant vectors comprising:
- a population of filamentous bacteriophage wherein each filamentous bacteriophage comprises a recombinant structural gene wherein each structural gene comprises an insert consisting of one member of an oligonucleotide population and wherein the population of filamentous bacteriophage comprise a plurality of different members of the oligonucleotide population,
- said oligonucleotide population comprising oligonucleotides comprising a coding region consisting of a length from about 4 to about 12 nucleotide triplets, said oligonucleotide population encoding a plurality of corresponding random peptide sequences from about 4 to about 12 L-amino acid residues, and
- wherein said recombinant structural genes are expressed upon transfer of said recombinant vectors into Escherichia coli host cells, and wherein expression of the recombinant structural genes yields polypeptides, each poly peptide comprising one of said plurality of corresponding random peptide sequences.
A method of producing a population of epitopic peptide sequences, comprising:
- Providing a population of recombinant Escherichia coli cells, each of said cells containing at least one member of a recombinant vector population, each member of said vector population comprising filamentous bacteriophage, wherein each filamentous bacteriophage comprises a recombinant structural gene wherein each structural gene comprises an insert consisting of one member of an oligonucleotide population and wherein the population of filamentous bacteriophage comprise a plurality of different members of the oligonucleotide population, said oligonucleotide population comprising oligonucleotides comprising a coding region consisting of a length from about 4 to about 12 nucleotides triplets, said oligonucleotide population encoding a plurality of epitopic peptides consisting of random sequences of from about 4 to about 12 L-amino acid residues:
- and
- Culturing said recombinant Escherichia coli cells to allow expression of said recombinant structural genes such that said epitopic peptide sequences are accessible to antibody recognition.
A population of recombinant vectors comprising substantially identical autonomously replicating nucleic acid sequences comprising a recombinant structural gene, each structural gene having inserted therein a member of an oligonucleotide population, wherein each member of said oligonucleotide population comprises a coding region comprising a length from about 4 to about 12 nucleotide triplets that encodes a corresponding peptide sequence of from about 4 to about 12 L-amino acid residues, and wherein the sum of corresponding peptide sequences encoded by said oligonucleotide population represents at least about 10% of all possible peptide sequences of said length, and wherein each member of said oligonucleotide population is contained in said recombinant vector population, and wherein the recombinant structural genes are expressed upon transfer of said recombinant vectors into Escherichia coli host cells, and wherein expression of said recombinant structural genes yields polypeptides, each polypeptide comprising said corresponding peptide sequence.
A method of producing a population of epitopic peptide sequences, comprising the steps of:
- Providing a population of recombinant E. coli cells, each of said cells containing at least one member of a recombinant vector population, each member of said vector population comprising substantially identical autonomously replicating nucleic acid sequences, said nucleic acid sequences comprising a recombinant structural gene, each structural gene having inserted therein a member of an oligonucleotide population wherein each member of said oligonucleotide population comprises a coding region comprising a length from about 4 to about 12 nucleotide triplets that encodes a corresponding peptide sequence of from about 4 to about 12 L-amino acid residues, and wherein each member of said oligonucleotide population is contained in said recombinant vector population and wherein the sum of said corresponding epitopic peptide sequences represents at least about 10% of all possible peptide sequences of said length:
- and culturing said recombinant E. coli cells to allow expression of said recombinant structural genes such that said epitopic peptide sequences are accessible to antibody recognition.
Dependencies are inferred from the claim text.