Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
AssigneeImmusol, Inc.
InventorsJack R. Barber, Peter Welch, Soonpin Yei, Richard Tritz
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Abstract
The present invention provides a hairpin ribozyme library having a randomized recognition sequence, packaged in a vector and operably linked to a promoter suitable for high level expression in a wide variety of cells. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.
Claims6
A method of identifying regulatory gene products and genes that control the expression of a particular selected gene, comprising the steps of:
- a. co-expressing in a same cell a selected reporter gene operably linked to a promoter of a selected gene and at least one member of a library of hairpin ribozyme-encoding nucleic acids having randomized recognition sequences, wherein a mRNA encoded by said reporter gene is not recognized or cleaved by a ribozyme expressed in said cell;
- b. identifying and cloning a cell wherein the level of expression of the reporter gene is measurably different from that of a cell that expresses the reporter gene but does not express said at least one member of the library of hairpin ribozyme-encoding nucleic acids;
- c. identifying a nucleic acid expressed in the cells cloned in step b whose gene product is recognized by a ribozyme expressed in said cloned cells.
An in vivo method of selecting at least one hairpin ribozyme that cleaves a target recognition site in a target nucleic acid, comprising the steps of:
- a. transducing a population of cells with a vector expressing a library of hairpin ribozyme-encoding nucleic acids having randomized recognition sequences, and with a nucleic acid that encodes at least one FACS-sortable reporter gene, under conditions that result in the expression of multiple different ribozymes per cell;
- b. selecting and cloning transduced cells that express at least one ribozyme-encoding nucleic acid whose gene product cleaves a target sequence in a selected target nucleic acid;
- c. isolating the ribozyme-encoding nucleic acids from the cloned cells of step b;
- d. packaging the ribozyme-encoding nucleic acids of step c;
- e. transducing a population of cells with the packaged ribozyme-encoding nucleic acids of step d;
- f. selecting and cloning transduced cells of step e that express at least one ribozyme-encoding nucleic acid whose gene product cleaves a target sequence;
- and
- g. isolating the ribozyme-encoding nucleic acid from the cloned cells of step f.
Dependencies are inferred from the claim text.