Vaccine for protection of poultry against salmonellosis and a process for preparing the same
AssigneeG.B. Pant University of Agriculture & Technology
InventorsVishwashwar Dutt Sharma, Subodh Kumar, Shri Krishna Garg, Ram Sagar Mishra, Tarani Kanta Barman
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Abstract
This invention relates to a process for the preparation of Salmonella vaccine by treating entrotoxin and cytotoxins with formalin and adding immuno-potentiator selected from Freund's complete adjuvant (FCA) or Vitamin E or Saponin to said concentrated toxoids to get the desired vaccine. The present invention also provides a Salmonella vaccine for protection against Salmonellosis in poultry.
Claims22
A process for the preparation of a vaccine from enterotoxin and cytotoxins isolated from Salmonella enterica subspecies for protection against Salmonellosis in poultry, comprising;
- treating the said toxins with formalin in a ratio appropriate to result in lost toxicity but retained immunogenicity and incubating for 40-50 hours at 35-38° C. temperature to form a formalized toxoid,
- removing formalin by dialysis against phosphate buffer saline (PBS),
- concentrating the formalized toxoid by dialysing against polyethylene glycol, and
- adding immuno-potentiator selected from Freund's Complete Adjuvant (FCA) or Vitamin E or Saponin, to said concentrated formalized toxoids to get the desired vaccine.
Dependencies are inferred from the claim text.