Nucleotide sequence for the Avian Metapneumovirus (Colorado) attachment glycoprotein gene
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsBruce S. Seal, Rene Alvarez
Life of this patent
Estimating the term…
Abstract
The nucleic acid and the corresponding amino acid sequence for the attachment glycoprotein of Avian Metapneumovirus (Colorado) Type C strain is provided. The nucleotide sequence is 1,321 base pairs with only one substantial open reading frame encoding a glycoprotein of about 435 amino acids with a predicted Mr of about 48,483 and a net charge of about 23.15 at neutral pH.
Claims3
An isolated nucleic acid sequence encoding an open reading frame of an avian pneumovirus (Colorado) Type C strain attachment glycoprotein having a sequence as set forth in SEQ ID NO 1.
A method for detecting avian pneumovirus (Colorado) Type C strain comprising:
- (a) isolating total RNA from a sample to be tested,
- (b) synthesizing a first strand DNA with SEQ ID NO. 1 from said isolated total RNA using a reverse primer selected from the group consisting of SEQ ID NO 3, SEQ ID NO 4, or mixtures thereof,
- (c) amplifying said first strand DNA using a primer from step b to form an amplified product, and
- (d) detecting the amplified first strand DNA product of step ( ).
Dependencies are inferred from the claim text.