T1 receptor-like ligand II and uses thereof
AssigneeHuman Genome Sciences, Inc.
InventorsJian Ni, Reiner L. Gentz, Steven M. Ruben
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel T1 Receptor (T1R)-like ligand II protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand II protein. T1R-like ligand II polypeptides are also provided, as are recombinant vectors and host cells for expressing the same. This invention further relates to pharmaceutical compositions and formulations comprising T1R-like ligand II. Also provided are methods of using T1R-like ligand II polynucleotides, polypeptides, antibodies or agonists/antagonists for therapeutic and diagnostic purposes. Diagnostic kits are further provided.
Claims58
A method of stimulating bone marrow cell proliferation, comprising contacting said cell with a protein comprising an amino acid sequence selected from the group consisting of:
- (a) amino acids 1 to 168 of SEQ ID NO:2;
- (b) amino acids 1 to 203 of SEQ ID NO:2;
- (c) amino acids −25 to 203 of SEQ ID NO:2;
- (d) amino acids −26 to 203 of SEQ ID NO:2;
- (e) the mature amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97655;
- and
- (f) the complete amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97655.
A method of stimulating bone marrow cell proliferation, comprising contacting said cell with a protein comprising an amino acid sequence at least 95% identical to a member selected from the group consisting of:
- (a) amino acids 1 to 168 of SEQ ID NO:2;
- (b) amino acids 1 to 203 of SEQ ID NO:2;
- (c) amino acids −25 to 203 of SEQ ID NO:2;
- (d) amino acids −26 to 203 of SEQ ID NO:2;
- (e) the mature amino acid sequence encoded by the eDNA contained in ATCC Deposit No. 97655;
- and
- (f) the complete amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97655;
- wherein said protein has the activity of stimulating the proliferation of bone marrow cells.
A method of stimulating bone marrow cell proliferation, comprising contacting said cell with a protein comprising an amino acid sequence which, except for one to five conservative amino acid substitutions, is identical to a member selected from the group consisting of:
- (a) amino acids 1 to 168 of SEQ ID NO:2;
- (b) amino acids 1 to 203 of SEQ ID NO:2;
- (c) amino acids −25 to 203 of SEQ ID NO:2;
- (d) amino acids −26 to 203 of SEQ ID NO:2;
- (e) the mature amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97655;
- and
- (f) the complete amino acid sequence encoded by the cDNA contained in ATCC Deposit No. 97655;
- wherein said protein has the activity of stimulating the proliferation of bone marrow cells.
A method of stimulating bone marrow cell proliferation comprising contacting said cell with a protein comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein said fragment has the activity of stimulating the proliferation of bone marrow cells.
A method of stimulating bone marrow cell proliferation comprising contacting said cell with a protein comprising a fragment of the complete amino acid sequence encoded by the eDNA clone contained in ATCC Deposit No. 97655, wherein said fragment has the activity of stimulating the proliferation of bone marrow cells.
Dependencies are inferred from the claim text.