Method of using perimeter measurements to improve ploidy analysis in a tissue section

Abstract

A method for correcting quantitative DNA measurements in a tissue section in which an operator measures, using an image analysis apparatus, the DNA content, profile area, and profile perimeter data for a plurality of nuclei and truncated nuclei in a Feulgen-stained histologic tissue section. The operator then operates a computer program embodying a method of analyzing ploidy measurements in a tissue section. The program includes a routine for obtaining an eccentricity index, which reflects the shape of each of the profiles of the nuclei and truncated nuclei in the tissue section. The program uses the eccentricity index of each nucleus and truncated nucleus to improve the accuracy of the calculation of the DNA content of the whole nuclei that existed in the tissue prior to making the tissue section, and to improve the discrimination of subpopulations of nuclei of different DNA content.

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