Transformation of hereditary material of plants
AssigneeSyngenta Investment Corporation
InventorsJerzy Paszkowski, Ingo Potrykus, Barbara Hohn, Raymond Douglas Shillito, Thomas Hohn, Michael William Saul, Vaclav Mandak
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Abstract
A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.
Claims10
A transgenic Brassica cell comprising foreign DNA unaccompanied by T-DNA border regions of a Ti plasmid, said foreign DNA being under the control of plant expression signals and integrated into the plant hereditary material, such that said foreign DNA is transmissible to the progeny of a plant regenerated from said transgenic plant cell, wherein said transgenic plant cell is transformed by a process for the stable integration of said foreign DNA comprising:
- contacting said foreign DNA directly with a plant protoplast in a medium under conditions that render the plant protoplast membrane permeable to DNA molecules until said foreign DNA has been taken up by the protoplast, wherein said stable integration is carried out by a technique selected from the group consisting of polyethylene glycol treatment, electroporation, heat shock treatment, and combinations thereof.
Dependencies are inferred from the claim text.