Agrobacterium-mediated plant transformation method
AssigneeMonsanto Company
InventorsCharles L Armstrong, Jyoti R Rout
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Abstract
The present invention relates to a novel transformation system for generating transformed plants with lower copy inserts and improved transformation efficiency. In particular, the invention relates to the use of Agrobacterium growth inhibiting agents during the Agrobacterium-mediated transformation process that suppress Agrobacterium growth and reduce T-DNA transfer to the target plant genome.
Claims6
A method of transforming a corn plant cell or plant tissue using an Agrobacterium mediated process comprising the steps of:
- inoculating a transformable plant cell or tissue from a corn plant with Agrobacterium containing at least one genetic component capable of being transferred to the plant cell or tissue in an inoculation media containing an effective amount of at least one antibiotic that inhibits or suppresses the growth of Agrobacterium;
- co-culturing the transformable plant cell or tissue after the inoculating step in a medium capable of supporting growth of plant cells or tissue expressing the genetic component, said medium not containing said antibiotic;
- selecting transformed plant cells or tissue;
- and
- regenerating a transformed corn plant expressing the genetic component from the selected transformed plant cells or tissue.
A method of transforming a plant cell or plant tissue using an Agrobacterium mediated process comprising the steps of:
- inoculating a transformable plant cell or tissue from a dicotyledonous plant with Agrobacterium containing at least one genetic component capable of being transferred to the plant cell or tissue in an inoculation media containing an effective amount of at least one antibiotic that inhibits or suppresses the growth of Agrobacterium;
- co-culturing the transformable plant cell or tissue after the inoculating step in a medium capable of supporting growth of plant cells or tissue expressing the genetic component, said medium not containing said antibiotic;
- selecting transformed plant cells or tissue;
- and
- regenerating a transformed plant expressing the genetic component from the selected transformed plant cells or tissue.
Dependencies are inferred from the claim text.