Method for regulating cell death
AssigneeNational Institute of Agrobiological Sciences
InventorsYuko Ohashi, Shigemi Seo
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Abstract
A method for regulating cell death in a plant, includes the steps of: transforming a plant cell with a polynucleotide containing a gene encoding DS9 or a homologue thereof or a part of the gene; and redifferentiating the transgenic plant cell to obtain a plant. The DS9 or the homologue thereof is an ATP-dependent Zn-type metalloprotease. The polynucleotide decreases or increases production of the ATP-dependent Zn-type metalloprotease in the plant cell, whereby cell death of a cell in the plant is promoted or suppressed.
Claims4
A method for promoting or suppressing a hypersensitive response in a plant, comprising the steps of:
- transforming a plant cell with a polynucleotide comprising a nucleic acid molecule encoding DS9 having the amino acid sequence of SEQ ID NO:1;
- and
- regenerating the transformed plant cell to obtain a plant, wherein the DS9 is an ATP dependent Zn-type metalloprotease, wherein said polynucleotide decreases or increases production of the ATP-dependent Zn-type metalloprotease in cells of the plant, whereby the hypersensitive response in the plant is promoted or suppressed, compared to an untransformed plant.
A method for producing a plant which has increased resistance to pathogens or superoxide generating herbicides, comprising the steps of:
- transforming a plant cell with a polynucleotide comprising a nucleic acid molecule encoding DS9 having the amino acid sequence of SEQ ID NO:1;
- and
- regenerating the transformed plant cell to obtain a plant, wherein the DS9 is an ATP-dependent Zn-type metalloprotease, wherein said polynucleotide decreases or increases production of the ATP-dependent Zn-type metalloprotease in cells of the plant, wherein resistance to said pathogens or said herbicides is increased compared to an untransformed plant.
Dependencies are inferred from the claim text.