Method of cloning animals
AssigneeInfigen, Inc.
InventorsNikolai S. Strelchenko, Jeffrey M. Betthauser, Gail L. Jurgella, Marvin M. Pace, Michael D. Bishop
Life of this patent
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Abstract
The present invention relates to cloning technologies. The invention relates in part to immortalized and totipotent cells useful for cloning animals, the embryos produced from these cells using nuclear transfer techniques, animals that arise from these cells and embryos, and materials, methods, and processes for establishing such cells, embryos, and animals.
Claims57
A method for preparing an ungulate animal, said method comprising:
- a) forming a first embryo by nuclear transfer of an ungulate cell obtained from a cell culture of a non-embryonic cell, or a nucleus thereof, into a first enucleated oocyte obtained from the same species as the ungulate cell;
- b) forming a second embryo by nuclear transfer of a cell obtained from said first embryo, or a nucleus thereof, into a second enucleated oocyte obtained from the same species as the cell in step (a) wherein said cell obtained from said first embryo is not expanded in culture;
- and
- c) transferring said second embryo into the uterus of an ungulate of the same species as the cell in step (a) so as to produce a fetus that undergoes full fetal development and parturition to generate said ungulate animal.
A method for reconstructing an ungulate embryo, said method comprising:
- a) forming a first embryo by nuclear transfer of an ungulate cell obtained from a cell culture, or a nucleus thereof, into a first enucleated oocyte obtained from the same species as the cell in;
- and
- b) forming a second embryo by nuclear transfer of a cell obtained from said first embryo, or a nucleus thereof, into a second enucleated oocyte obtained from the same species as the cell in step (a).
A method for preparing an ungulate animal, said method comprising:
- a) forming a first embryo by nuclear transfer of an ungulate cell obtained from a first cell culture of a non-embryonic cell, or a nucleus thereof, into a first enucleated oocyte obtained from the same species as the ungulate cell;
- b) transferring said first embryo into the uterus of an ungulate of the same species as the cell in step (a) so as to produce a first fetus;
- c) forming a second cell culture by culturing one or more cells obtained from said first fetus;
- d) forming a second embryo by nuclear transfer of a cell obtained from said second cell culture, or a nucleus thereof, into a second enucleated oocyte obtained from the same species as the cell in step (a);
- and
- e) transferring said second embryo into the uterus of an ungulate of the same species as the cell in step (a) so as to produce a second fetus that undergoes full fetal development and parturition to generate said ungulate animal.
Dependencies are inferred from the claim text.