Mammalian prestin polynucleotides
AssigneeNorthwestern University
InventorsPeter Dallos, Jing Zheng, Laird D. Madison
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Abstract
The invention relates to mammalian prestin protein, which has been discovered to be the mammalian cochlear outer hair cell motor, and to polynucleotides encoding prestin. Full length gerbil prestin and its cDNA are described, full length murine prestin and its cDNA are described, and a partial sequence of human prestin and its chromosomal location are described.
Claims6
An isolated polynucleotide consisting of 22 to 400 consecutive nucleotides of the murine prestin cDNA sequence set forth in SEQ ID NO:4, wherein said polynucleotide hybridizes with high stringency with the coding region of a mammalian prestin nucleic acid under the following hybridization conditions at about 50 degrees Celsius:
- a. 0.015 M NaCl;
- b. 1.5 mM sodium citrate;
- and
- c. 0.1% (w/v) sodium dodecyl sulfate.
An isolated polynucleotide comprising the coding region of a mammalian prestin nucleic acid, wherein the coding region is at least 87% homologous with SEQ ID NO:4, and further wherein said isolated polynucleotide encodes a polypeptide that modulates the shape of mammalian cochlear outer hair cells in response to the membrane potential of the plasma membrane of the cells.
An isolated polynucleotide comprising the sequence listed in SEQ ID NO:4.
An isolated polynucleotide consisting of 22 to 400 consecutive nucleotides of the murine prestin cDNA sequence set forth in SEQ ID NO:4, wherein said polynucleotide hybridizes with high stringency with the coding region of a mammalian prestin nucleic acid under the following hybridization conditions at about 42 degrees Celsius:
- a. 50% (v/v) formamide;
- b. 0.1% (w/v) bovine serum albumin;
- c. 0.1% (w/v) Ficoll;
- d. 0.1% (w/v) polyvinylpyrrolidone;
- e. 50 mM sodium phosphate buffer at pH 6.5;
- f. 750 mM NaCl;
- and
- g. 75 mM sodium citrate.
Dependencies are inferred from the claim text.